GENTAUR BVBA
VAT BE0473327336
Av. de l Armee 68 B4
1040 Brussels
BELGIUM
Tel + 32 16 58 90
45
Fax + 32 16 50 90 45
GENTAUR France SARL
SIRET 48423788800017
Rue Lagrange, 9
75005 Paris,
France
Tel 01 43 25 01 50
Fax 01 43 25 01 60
GENTAUR Germany
52074 Aachen,
Germany
Tel
0241 40 08 90 86
Fax 0241 55 91
05 36
GENTAUR
Pol Sp. Z.o.o. Ulica
Ogarna 15/19B m2
80-826 GDANSK
Tel 00 48 51 760 77
08
Fax: 00 32 16 50 90
45
GENTAUR Italy
23015 Milano, Italy
Tel 02 36 00 65
93
Fax 02 36 00 65
94
Česká republika
Praha
+420246019719
Danmark
+4569918806
Finland Helsset
+358942419041
Ελλάς Αθήνα
+302111768494
Ireland Dublin
+35316526556
Luxembourg
+35220880274
Magyarország
Budapest
+3619980547
Nederland
+31208080893
Norge
Oslo
+4721031366
Österreich
+43720880899
Sverige
Stockholm
+46852503438
Schweiz Züri
+41435006251
Northern America
Canada Montreal
+15149077481
US New York
+17185132983
Other Countries
0032 (0)16 41 44 07 |
|
|
|
|
|
|
| |
APO ACTIVE 3
Phycoerythrin (PE)
Antibody Specific Active Caspase 3 Detection Kit
|
Key Benefits: |
-
Highly specific for active human and murine caspase 3.
Other assays require the utilization of peptide based (DEVD)
reagents that tend to cross-react with caspase 7 and
other caspases.
-
Applications - Works with a flow cytometer,
flourescence microscope, or 96 well plate reader.
-
Yields both quantitative and qualitative results. Gives
a strong positive signal.
-
Can be used in conjunction with other antibodies or
stains.
-
No need to make cell lysates or run western blots. Cells
can be fixed and analyzed later.
-
Works with human, mouse and rat cell lines.
|
Assay Principle: |
Cell Technology ’s APO-ACTIVE 3 PE Kit utilizes a rabbit
affinity purified polyclonal antibody raised against amino
acid 163-175 of murine caspase 3 (9). This neo epitope is
present on the p18 subunit of cleaved caspase 3 (9). Cells
undergoing apoptosis are fixed and permeablized prior to the
addition of the antibody. A secondary PE labeled goat anti
rabbit antibody is used to visualize the bound rabbit anti
caspase 3 polyclonal. PE labeling allows for use with a
variety of applications.
|
References: |
-
Slee, E. A., C. Adrain, and S. J. Martin. 1999. Serial
Killers: orderingcaspase activation events in apoptosis.
Cell Death and Differ. 6:1067-1074.
-
Walker, N. P., R. V. Talanian, K. D. Brady, L. C. Dang,
N. J. Bump, C. R.Ferenz, S. Franklin, T. Ghayur, M. C.
Hackett and L. D. Hammill. 1994. Crystal Structure of
the Cysteine Protease Interleukin-1ß-Converting Enzyme:
A(p20/p10)2
Homodimer. Cell 78:343-352.
-
Wilson, K. P., J. F. Black, J. A. Thomson, E. E. Kim, J.
P. Griffith, M. A.Navia, M. A. Murcko, S. P. Chambers,
R. A. Aldape, S. A. Raybuck, and D. J.Livingston. 1994.
Structure and mechanism of interleukin-1 beta converting
enzyme. Nature 370: 270-275.
-
Rotonda, J., D. W. Nicholson, K. M. Fazil, M. Gallant,
Y. Gareau, M. Labelle,E. P. Peterson, D. M. Rasper, R.
Ruel, J. P. Vaillancourt, N. A. Thornberry and J.W.
Becker. 1996. The three-dimensional structure of apopain/CPP32,
a key mediator of apoptosis. Nature Struct. Biol. 3(7):
619-625.
-
Kumar, S. 1999. Mechanisms mediating caspase activation
in cell death. Cell Death and Differ. 6: 1060-1066.
-
Alnemri, E.S. et al (1996) Cell 87:171
-
Trends Biochem Sci 22,388 (1997)
-
Nature 376, 37 (1995).
|
Kit contents: |
|
The
following kits are available: |
Product
|
Catalog No.
|
Size
(No. of Tests) |
Price (US$) |
APO ACTIVE 3 PE
Antibody Specific Active Caspase 3 Detection Kit |
PAB200-1 |
25 |
€145 |
PAB200-2
|
100 |
€425 |
|
|
(A)
|
(B) |
|
|
|