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redcoon België GENTAUR BVBA

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  Tel + 32 16 58 90 45 

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APO ACTIVE 3 Phycoerythrin (PE) ™
Antibody Specific Active Caspase 3 Detection Kit
 
Key Benefits:
  • Highly specific for active human and murine caspase 3. Other assays require the utilization of peptide based (DEVD) reagents that tend to cross-react with caspase 7 and other caspases.
  • Applications - Works with a flow cytometer, flourescence microscope, or 96 well plate reader.
  • Yields both quantitative and qualitative results. Gives a strong positive signal.
  • Can be used in conjunction with other antibodies or stains.
  • No need to make cell lysates or run western blots. Cells can be fixed and analyzed later.
  • Works with human, mouse and rat cell lines.
Assay Principle:
Cell Technology ’s APO-ACTIVE 3 PE Kit utilizes a rabbit affinity purified polyclonal antibody raised against amino acid 163-175 of murine caspase 3 (9). This neo epitope is present on the p18 subunit of cleaved caspase 3 (9). Cells undergoing apoptosis are fixed and permeablized prior to the addition of the antibody. A secondary PE labeled goat anti rabbit antibody is used to visualize the bound rabbit anti caspase 3 polyclonal. PE labeling allows for use with a variety of applications.
Kit contents:
  • 1 vial of Rabbit anti cleaved caspase 3 affinity purified polyclonal antibody
  • 1 vial of PE labeled Goat anti Rabbit affinity purified polyclonal antibody
  • 1 vial of 10X fixative.
The following kits are available:
Product
 
Catalog No.
 
Size
(No. of Tests)
Price (Eur)
APO ACTIVE 3 PE Antibody Specific Active Caspase 3 Detection Kit PAB200-1 25 €145
PAB200-2
 
100 €425
 

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