Cell Technology ’s APO-ACTIVE 3 PE Kit
utilizes a rabbit affinity purified
polyclonal antibody raised against amino
acid 163-175 of murine caspase 3 (9). This
neo epitope is present on the p18 subunit of
cleaved caspase 3 (9). Cells undergoing
apoptosis are fixed and permeablized prior
to the addition of the antibody. A secondary
PE labeled goat anti rabbit antibody is used
to visualize the bound rabbit anti caspase 3
polyclonal. PE labeling allows for use with
a variety of applications.
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