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RNAI Libraries
RNAi Clone Library targeting all annotated genes in the human and mouse genomes.



Recent work has identified that a conserved biological response to double-stranded RNA, known as RNA interference (RNAi), specifically silences protein-coding genes through degradation of homologous mRNAs. Scientists at Cold Spring Harbor Laboratory (CSHL) have exploited this natural process to efficiently and cost-effectively probe gene function through targeted RNAi-induction.

RNAi Clone Library:

Scientists in the Hannon Lab at CSHL are generating an RNAi Clone Library consisting of at least 3 siRNAs specifically targeting every annotated gene in the human and mouse genomes. By allowing for selective silencing of any gene in the genome, these large-scale libraries of RNAi-inducing plasmids provide a powerful tool for systematically probing gene function on a whole genome scale and introduce novel methods to screen for potential therapeutic targets associated with diseases ranging from HIV to cancer.

Greg Hannon is an expert in the areas of post-transcriptional gene silencing and RNAi. His laboratory has been responsible for many of the seminal discoveries that uncovered the role of RNAi in normal cell physiology. For a more detailed explanation of RNAi and the potential of this technology please refer to his most recent articles “Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells.” (Genes Dev. 2002; 16, 948-958), “Germline transmission of RNAi” (Nat Struct Biol. 2003; 10, 91-92) and “An epi-allelic series of p53 hypomorphs created by stable RNAi produces distinct tumor phenotypes in vivo.” (Nat Genet. 2003; 33, 396-400).


The ability to generate RNAi-inducing clones individually target specific genes in the human genome will permit rapid, cost-efficient, loss-of -function genetic screens and rapid tests for genetic interactions to be performed in mammalian cells. Such approaches hold tremendous promise for unleashing the dormant potential of sequenced genomes and provide drug companies and individual researchers with an efficient means to help locate gene targets involved in any disease of interest.