Home Up Call me back Contents Contact Immunology / Infectious agents / Cellular Biology / Molecular biology / Instruments / Lentiviral Technology / RNAi Libreries / Stem Cell Research / MicroRNA / Proteomics / Chemicals / Campaign / Antibodies Myeloperoxidase Detection 

Google

       Pricelist 2010 

Home
Up

redcoon België GENTAUR BVBA

VAT BE0473327336

Av. de l Armee 68 B4

1040 Brussels BELGIUM

  Tel + 32 16 58 90 45 

Fax + 32 16 50 90 45

GENTAUR France SARL

SIRET 48423788800017

Rue Lagrange, 9

75005 Paris, France

 Tel 01 43 25 01 50

Fax 01 43 25 01 60 

GENTAUR Germany

52074 Aachen, Germany

 Tel 0241 40 08 90 86

Fax 0241 55 91 05 36

GENTAUR Pol Sp. Z.o.o. Ulica Ogarna 15/19B m2

80-826 GDANSK

Tel 00 48 51 760 77 08

Fax: 00 32 16 50 90 45

GENTAUR Italy

23015 Milano, Italy

 Tel 02 36 00 65 93

Fax 02 36 00 65 94

Česká republika Praha
+420246019719
 

Danmark

+4569918806 

Finland Helsset
+358942419041

Ελλάς Αθήνα
+302111768494
 

Ireland Dublin
+35316526556
 

Luxembourg
+35220880274
 

Magyarország Budapest
+3619980547
 

Nederland
+31208080893
 

Norge Oslo
+4721031366
 

Österreich
+43720880899
 

Sverige Stockholm
+46852503438
 

Schweiz Züri
+41435006251
 

 

Northern America 

Canada Montreal
+15149077481
 

US New York
+17185132983

 

Other Countries redcoon België
0032 (0)16 41 44 07

 

Fluoro MPOHOCL
Fluorescent Myeloperoxidase  Chlorination and peroxidation Detection Kit
 
Key Benefits:
  • Can monitor multiple time points to follow kinetics.
  • One-step, no wash assay.
  • Adaptable for High Throughput format
  • Highly Sensitive
  • Applications - Fluorescence Plate Reader
Assay Principle

Myeloperoxidase (MPO) is a highly cationic glycosolated hemoprotein that has a molecular weight of 144kD. The hemoprotein consists of two dimers linked via a disulfide bridge. Each dimmer is composed of a heavy (53kD) and light (15kD) subunit. Each heavy chain contains an independently acting protoporphyrin group containing a central iron (1-5). MPO is present in the azurophilic granules of polymorphonuclear leukocytes (PMNs) and is unique to neutrophils and monocytes. However, monocytes contain only one third of the MPO found in PMN’s. MPO utilizes H202 produced by the neutrophils to oxidize a varity of aromatic compounds to give substrate radicals for bactericidal activity (4 review). This enzyme is unique however in that it can oxidize chloride ions to produce a strong nonradical oxidant,HOCl. HOCl is the most powerful bactericidal produced by neutrophils (4 review). Excessive production of these radicals can cause oxidative stress leading to oxidative tissue injury.

Chlorination Reaction:

H2O2 + MPO + Cl- ----> HOCl + APF (non fluorescent) ----> Fluorescent Dye
                                          
Excitation:488nm; Emission: 515-530nm

Peroxidation Reaction:

H2O2 + Detection reagent (non-fluorescent)+ MPO ----->   fluorescent analog

 Excitation 530-571nm Emission  590-600nm

Applications
  • Detection of MPO chlorination activity in neutrophils and macrophages.
  • Detection of PMN infiltration in tissue samples (inflammation and innate host defense mechanisms).  
  • Acute and chronic inflammatory disorders due to chlorination tissue damage.  
  • MPO chlorination activity in acute and chronic manifestations of cardiovascular disease.
Kit contents (for 500 assays of Myeloperoxidase detection and 300 assays of Chlorination detection)
  1. 10X Assay Buffer: 60ml 
  2. Detection reagent: One vial for 500 assays.
  3. Hydrogen Peroxide: 1000µL of a stabilized 3% solution.
  4. Myeloperoxidase: 1 vial at 30 units/ml
  5. 1 vial of APF

 

Peroxidase and Chlorination Detection
Catalog
 
Size
 
Price (Eur)
MPOHOCL 100-3
 
500 + 300 Tests €590