1. Equilibrate kit to room temperature |
- |
2. Add
samples and standards to wells |
2 hours room
temp. (or overnight 4°C) |
4. Add
detector antibody to all wells |
2 hours -
room temp. |
6. Add HRP conjugate to all wells |
30 minutes -
room temp. |
8. Add substrate to all wells |
30 minutes -
room temp. |
9. Add stop
solution to all wells |
- |
10. Read
plate at 450 nm/550 nm |
- |