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Introducing Ziva™ Ultrasensitive BrdU Cell Proliferation Assay Research Use Only Potential Uses Cancer - Detection of proliferating tumor cells or metastatic cells in blood- Detection of tumor-specific T-cells- Detection of stimulatory or inhibitory/toxic effects of various compounds/drug candidates on tumor or normal, primary or cell line viabilityImmunology - Detection of immunity using small numbers of cells- Early detection of cell proliferation- Detection of antigen specific T-cell proliferation- Detection and quantification of the immunoreactivity of lymphocytes, stimulatory mitogens or antigens, after exposure to various cytokines or growth factors. Antigen specificity.Autoimmunity/Transplantation - Detection of autoreactive T-cells- Comparison of responses of donor vs host samplesRecombinant Expression Technology - Detection of transfected cellsStem Cells - Quality assessment of stem cells under various media conditionsThe Ziva Performance Difference - 200 x more sensitive than 3H-thymidine (the "Gold Standard")- A broader dynamic range than 3H-thymidine- A lower limit of detection: 1-4 proliferating cells in a background of 100,000 viable non-proliferating cells- Simple to use, non-isotopic, 96 microtiter plate, ELISA format- Assay performance time: < 1 hour- Flexibility in small sample size (1 to 105 cells) and sample volume (50 μL to 100 μL), are ideal for longitudinal in vivo studies in rodents. |
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