| Conjugate
Diluents |
| Improves Assays:
|
-
Increases the Sensitivity of the Assay.
-
Decreases Backgrounds.
-
Stabilizes Newly Conjugated Proteins. Also
reconstitutes lyophilized conjugates and diluents concentrates
conjugates.
-
Prolongs the Shelf-Life of HRP-conjugated
proteins and enhancing their utility in ELISA applications.
-
Extends the Useful Titer of the conjugate.
-
Conserves Valuable Reagents. By promoting a
higher specific signal, less conjugate may be needed to generate
a signal when a stabilizing conjugate diluent is used.
-
Lengthens the Shelf-Life of conjugated
antibodies for future use. With a longer shelf-life, conjugates
may be prepared in batches for use in future plate coating runs.
Using conjugates from the lot generally reduces inter-assay
variability and increases consistency.
|
| How it Works: |
|
Conjugate Diluents stabilize newly conjugated proteins, reconstitute
yophilized conjugates, and dilute concentrated conjugates. Cell
Technology's conjugate diluents stabilize the immunoglobulin and HRP
components of the conjugate - protein complex. All conjugate
diluents are available at 1x and 5x concentrations. When stored
unopened and protected from light, a shelf-life of 18 months can be
expected at 2°-8°C.
|
| Directions: |
|
To stabilize newly conjugate proteins, add 1 part of the appropriate
5x conjugate diluent (that has not been diluted to 1x) to 4 parts of
the new conjugate solution.
To reconstitute lyophilized conjugates, simply pipette a known
amount of a 1x conjugate diluent into the vial. The liquid conjugate
is now ready to be used directly, or further diluted to the required
concentration.
To dilute concentrated conjugates for use in an assay, pipette a
known amount of the conjugate stock into a 1x conjugate diluent.
|
| ECD100
Monoclonal- Polyclonal Conjugate Diluent & Stabilizer |
|
Monoclonal-Polyclonal Conjugate Diluent & Stabilizer is a unique
formulation specifically designed for use in mouse monoclonal
capture and polyclonal-HRP sandwich ELISA formats. This diluent
contains mouse proteins which suppress nonspecific binding of the
polyclonal-HRP conjugate to the mouse immunoglobulins adsorbed onto
the plate. These protein additives also provide additional
structural stability to the conjugate protein complex. Other
additives contained in ECD100 serve to extend the useful titer of
the conjugate by stabilizing both the immunoglobulin and HRP
components of the conjugate. Antimicrobial agents have also been
added to retard bacterial growth. When stored unopened and protected
from light, a shelf-life of 18 months can be expected at 2°-8°C.
|
| 5x Monoclonal - Polyclonal Diluent &
Stabilizer (ECD105) |
Catalog Number
|
final volume at 1x
|
Bottle size (mL)
|
#Plates at 100uL/well 1x
(9.6mL/plate) |
#Plates at 200uL/well
(19.2mL/plate) |
Price |
| ECD105-1 |
500 mL |
100 |
50 |
25 |
€220.00 |
| ECD105-2 |
2.5L
|
500 |
255 |
126 |
€695.00 |
| ECD105-3 |
5L |
1000 |
515 |
255 |
€1175.00 |
| ECD105-4 |
50L |
10000 |
5200 |
2600 |
€10900.00 |
|
| ECD200 Antigen-Down Conjugate
Diluent & Stabilizer |
|
Antigen-Down Conjugate Diluent & Stabilizer is a unique formulation
specifically designed for use with anti-IgG-HRP conjugates. This
diluent contains proprietary non-mammalian protein additives which
will not interfere with anti-IgG-HRP conjugates used in antigen-down
and sandwich ELISA formats. These additives also provide additional
structural stability to the IgG-HRP conjugate protein complex. CD2
will also inhibit conjugate binding to nonspecific serum proteins
bound to the plate, thus reducing conjugate bridging on the plate
surface and lowering background noise. Antimicrobial agents have
been added to retard bacterial growth. When stored unopened and
protected from light, a shelf-life of 18 months can be expected at
2°-8°C.
|
| 5x Antigen- Down
Conjugate Diluent & Stabilizer (ECD205) |
Catalog Number
|
Final volume at 1x
|
Bottle size (mL)
|
Plates at 100uL/well 1x (9.6mL/plate)
|
Plates at 200uL/well 1x (19.2mL/plate) |
Price |
| ECD205-1 |
500mL
|
100
|
50 |
25 |
€220.00 |
ECD205-2
|
2.5L |
500 |
255 |
126
|
€695.00 |
ECD205-3
|
5L |
1000 |
512 |
255 |
€1175.00 |
| ECD205-4 |
50L |
10000 |
5200 |
2600 |
€10900.00 |
|
| ECD300 Polyclonal-Polyclonal
Conjugate Diluent & Stabilizer |
|
Polyclonal-Polyclonal Conjugate Diluent & Stabilizer is a unique
formulation specifically designed for use in polyclonal capture and
polyclonal-HRP sandwich ELISA formats. Proprietary additives were
specifically selected to provide optimal assay performance when
using rabbit, goat, or rabbit and goat polyclonal antibodies in a
sandwich ELISA format. ECD300 contains a uniquely processed form of
rabbit and goat IgG to suppress nonspecific binding of the
polyclonal-HRP conjugates to the plate surface. To reduce
nonspecific binding interactions, a detergent is also included.
Antimicrobia agents have been added to retard bacterial growth. When
stored unopened and protected from light, a shelf-life of 18 months
can be expected at 2°-8°C.
|
| 5x Polyclonal -
Polyclonal Conjugate Diluent & Stabilizer (x CD3) |
Catalog Number
|
Final volume at 1x
|
Bottle size (mL) |
#Plates at 100uL/well 1x (9.6mL/plate)
|
#Plate at 200uL/well 1x (19.2mL/plate) |
Price |
ECD305-1
|
500mL
|
100 |
50 |
25 |
€490.00 |
ECD305-2
|
2.5L |
500 |
255 |
126 |
€1995.00 |
| ECD305-3 |
5L |
1000 |
515 |
255 |
€3700.00 |
| ECD305-4 |
50L |
10000
|
5200 |
2600 |
€34500.00 |
|
| ECD400 Monoclonal - Goat
Polyclonal Conjugate Diluent & Stabilizer |
|
This diluent is a unique formulation specifically designed for use
in monoclonal capture and goat polyclonal-HRP sandwich ELISA
formats. Additives were carefully selected to specifically address
the stability and nonspecific binding issues associated with this
type of ELISA format. Goat proteins are included to reduce any
nonspecific interactions between the monoclonal capture and goat
polyclonal-HRP antibodies. CD4 also contains mouse IgG to
specifically suppress nonspecific bridging of the goat polyclonal-HRP
conjugate with the mouse capture IgG on the plate surface. To
further reduce nonspecific binding of the conjugate to the plate
surface, adetergent is also included. Antimicrobial agents have been
added to retard bacterial growth. When stored unopened and protected
from light, a shelf-life of 18 months can be expected at 2°-8°C.
|
| 5x Monoclonal - Goat
Polyclonal HRP Conjugate Diluent & Stabilizer (ECD405) |
Catalog Number
|
Final volume at 1x
|
Bottle Size (mL)
|
#Plates at 100uL/well 1x
(9.6mL/plate)
|
#Plates at 200uL/well 1x
(19.2mL/plate)
|
Price |
ECD405-1
|
500ml
|
100 |
50
|
25 |
€295.00 |
| ECD405-2 |
2.5L |
500 |
255 |
126 |
€895.00 |
| ECD405-3 |
5L |
1000 |
515
|
255
|
€1495.00 |
| ECD405-4 |
50L |
10000 |
5200 |
2600 |
€12995.00 |
|
| ECD600 Conjugate Stock
Stabilizing Reagent |
Conjugate Stock Stabilizing Reagent contains a BSA-based mammalian
protein additive to stabilize conjugated proteins, and magnesium and
calcium salts to stabilize the catalytic site of the HRP porphyrin
ring structure. When diluted to 1x, ECD600 is an excellent HRP
conjugate diluent and stabilizer base, to which additional
components can be added to for use in your specific assay.
At 5x, ECD600 is an excellent diluent for stabilizing newly
conjugated proteins. This allows the liquid conjugate stock to be
stored, and then later diluted to make a working conjugate for use
in the assay. Simply add 1 part of 5x CD6 (that has not been diluted
to 1x) to 4 parts of the new conjugate solution. For example, if you
just eluted 20 mL of an antibody-HRP conjugate off a column, simply
add 5 mL of 5x CD6 to the conjugate solution. If the conjugate was
initially at 10 mg/mL, the stabilized conjugate will now be at 8 mg/mL
in 25mL diluent (it's been diluted by 20%).
Antimicrobial agents have also been added to ECD6 retard
bacterial growth. When stored unopened and protected from light, a
shelf-life of 18 months can be expected at 2°-8°C.
|
| 5x Conjugate Stock
Stabilizing Reagent (ECD605) |
Catalog Number
|
Final volume at 1x
|
Bottle size (mL)
|
#Plates at 100uL/well 1x
(9.6mL/plate)
|
#Plates at 200uL/well 1x
(19.2mL/plate)
|
Price |
ECD605-1
|
500mL
|
100 |
50
|
25 |
€195.00 |
| ECD605-2 |
2.5L |
500 |
255
|
125 |
€595.00 |
| ECD605-3 |
5L |
1000 |
515 |
255 |
€1095.00 |
| ECD605-4 |
50L |
10000 |
5200 |
2600 |
€8895.00 |
|
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