Plate Coating Buffers

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Universal Plate Coating Buffer

Improves Assays:

Saves valuable reagents. By promoting a higher specific signal, less protein may be needed to coat the plate and less of the detection molecules may be needed to generate a signal when this buffer is used.
Increases the shelf-life of your plates. Depending on the activity of the coated protein, plates may be stored at room temperature or 2°-8°C for several months, or even years under proper conditions.
Increases reproducibility. By using consistent reagents from a reliable source, your ELISAs may become more reproducible.
Increases the signal of the ELISA. If the antibody or antigen is not sticking to the plate, or unwanted proteins are interfering with binding, the plate will have an overall low specific signal. CB1 may foster adsorption and reduce interference.
Decreases background noise. If excess proteins are sticking to the plate, plate will have overall high backgrounds (a high signal overall, but a low specific signal). Universal Plate Coating Buffer may reduce interference.

How it Works:

Our Coating Buffer is an optimal formulation of buffers and salts which maximizes the adsorption of antibodies and antigens onto polystyrene plates. During the adsorption process, the Coating Buffer stabilizes coated proteins by maintaining their tertiary three-dimensional structure. By stabilizing the adsorbed protein, antigenic regions are preserved, allowing for greater binding reactivity with the detection molecule, thereby enhancing the specific signal. By generating a higher specific signal, a lower concentration of coat protein may be needed when this buffer is used, thereby saving valuable reagents.

Directions:

Simply dilute Coating Buffer 1:5 (for example, add 100mL Coating Buffer to 400mL diH20 for a final volume of 500mL) , add your protein antigen or antibody, let the solution stir for 15 minutes, and pipette onto the plate.

ECB100 Universal Plate Coating Buffer

Cell Technology has specifically formulated a unique plate coating buffer for use with antibodies and antigens. The pH and ionic strength of this buffer have been specifically formulated to create an optimal coating environment which maximizes the adsorption efficiency of most antibodies and protein antigens onto polystyrene plate surfaces. This buffer also stabilizes coated proteins, preserving antigenic regions, and allowing for greater binding reactivity with the detection molecule, enhancing the specific signal. By generating a higher specific signal, a lower concentration of coat protein may be needed when this buffer is used, thereby saving valuable reagents.

5x Universal Plate Coating Buffer (ECB100)
5x Bottle Size	Price per Bottle	Part Number	Volume at 1x	Plates at 50uL/well (4.8mL/plate)	Plates at 200uL/well (9.6mL/plate)	Plates at 300uL/well (28.8mL/plate)
100 mL	€40	644	500mL	100	25	16
500 mL	€95	645	2.5L	510	125	84
1 L	€195.00	646	5L	1020	250	170
10 L	€1350.00	658	50L	10,200	2,500	1,700