GENTAUR BVBA
VAT BE0473327336
Av. de l Armee 68 B4
1040 Brussels
BELGIUM
Tel + 32 16 58 90
45
Fax + 32 16 50 90 45
GENTAUR France SARL
SIRET 48423788800017
Rue Lagrange, 9
75005 Paris,
France
Tel 01 43 25 01 50
Fax 01 43 25 01 60
GENTAUR Germany
52074 Aachen,
Germany
Tel
0241 40 08 90 86
Fax 0241 55 91
05 36
 GENTAUR
Pol Sp. Z.o.o. Ulica
Ogarna 15/19B m2
80-826 GDANSK
Tel 00 48 51 760 77
08
Fax: 00 32 16 50 90
45
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23015 Milano, Italy
Tel 02 36 00 65
93
Fax 02 36 00 65
94
Česká republika
Praha
+420246019719
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+4569918806
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+358942419041
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Other Countries
0032 (0)16 41 44 07 |
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| Sample Diluents |
| Improves Assays: |
| By accurately reading and quantitating high-titer
samples. High-titer samples may overload the finite binding capacity of
the coated ELISA plate surface, thus requiring dilution prior to testing
in the assay. |
| Diluents Available: |
-
ESD100 General-Purpose
Sample Diluent - All ELISAs For dilution of most serum, ascites,
cell-culture supernatants, and antigens into the functional
range of the ELISA.
-
ESD200 Plasma Sample Diluent
- Antigen-Down For dilution of plasma samples into the
functional range of the antigen-down ELISA to inhibit complement
and thrombin (clotting) activity during the assay.
-
|
| Directions: |
|
Simply multiply the value determined from the curve by your dilution
factor. For example, if you diluted your sample 1:2 (250uL sample
into 250uL sample diluent) and this yielded an OD of 0.437, when
read off the curve, the OD of 0.437 correlated to 200 pg/mL, you
would simply multiply 200pg/mL by 2 (your dilution factor) yielding
a value of 400pg/mL, meaning that the original starting sample had
400pg/mL of analyte present.
|
| ESD100 General-Purpose
Sample Diluent - All ELISAs |
|
General-Purpose Sample Diluent is formulated for diluting goat,
rabbit, mouse, and human sera, as well as cell culture media and
mouse ascites fluid. ESD100 can also be used for dilution of
mammalian and chicken serum samples in antigen-down ELISA formats.
Hyperimmunized serum samples contain nonspecific IgG antibody that
may interfere with the titration. Proprietary additives have been
incorporated into this diluent that minimize nonspecific binding
interactions of the non specific IgG in the samples, thus decreasing
background noise. Dilutions of the antigen standard for use in
sandwich ELISA formats have also been carried out using this diluent.
ESD100 contains a buffered BSA protein base to assure a constant pH
and solute environment favorable to antibody-antigen interactions.
It also contains an antimicrobial agent to provide a solution free
of bacterial contamination.
|
| General-Purpose Sample
Diluent (ESD100)
|
Catalog Number
|
Size (mL)
|
#Samples diluted 1:2 using 250uL
|
#Samples diluted 1:10 using 450uL |
Price |
| ESD100-1 |
100 |
400 |
220 |
€55.00 |
| ESD100-2 |
500 |
2,000 |
1,100 |
€165.00 |
| ECD100-3 |
1,000 |
40,00 |
2,200 |
€265.00 |
| ECD100-4 |
10,000 |
40,000 |
22,200 |
€2,100.00 |
|
| ESD200 Plasma Sample Diluent - Antigen-Down |
|
Plasma Sample Diluent is formulated specifically for use with plasma
samples being tested in an antigen-down ELISA format. This diluent
contains proprietary additives in a buffered BSA protein base with
which provide an assay environment that minimizes nonspecific
binding while maintaining proper ionic strength and pH for efficient
antibody-antigen interaction. Additional additives have been
included to inhibit thrombin (clotting) and complement activity
during the plasma sample incubation period. ESD200 also contains an
antimicrobial agent to provide a solution free of bacterial
contamination.
|
| Plasma Sample Diluent -
Antigen-Down (ESD200)
|
Catalog Number
|
Size (mL)
|
#Samples diluted 1:2 using 250uL
|
#Samples diluted 1:10 using 450uL |
Price |
| ESD200-1 |
100 |
400 |
220 |
€75.00 |
| ESD200-2 |
500 |
2,000 |
1,100 |
€230.00 |
| ECD200-3 |
1,000 |
40,00 |
2,200 |
€365.00 |
| ECD200-4 |
10,000 |
40,000 |
22,200 |
€2,900.00 |
|
| ESD300 Nonmammilian Sample Diluent |
|
Nonmammilian Sample Diluent contains proprietary additives in a
buffered BSA protein base with which provide an assay environment
that minimizes nonspecific binding while maintaining proper ionic
strength and pH for efficient antibody-antigen interaction. ESD300
also contains an antimicrobial agent to provide a solution free of
bacterial contamination.
|
| Pisces Sample Diluent -
(ESD300) |
Catalog Number
|
Size (mL)
|
#Samples diluted 1:2 using 250uL
|
#Samples diluted 1:10 using 450uL |
Price |
| ESD300-1 |
100 |
400 |
220 |
€75.00 |
| ESD300-2 |
500 |
2,000 |
1,100 |
€230.00 |
| ECD300-3 |
1,000 |
40,00 |
2,200 |
€365.00 |
| ECD300-4 |
10,000 |
40,000 |
22,200 |
€2,900.00 |
|
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