TRAIL-R1/Fc Chimera

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Recombinant human TRAIL-R1 (DR4/ TNFRSF10A)/Fc Chimera

Source:

Produced in HEK 293 cells. The extracellular domain of human TRAIL-R1 (aa 1 - 238) is fused to the Fc portion of human IgG1.

Description:

DR4 is a transmembrane protein, also called TRAIL receptor 1 (TRAIL-R1). The ligand for this DR4 death receptor has been identified and termed TRAIL, which is a member of the TNF family. DR4, as many other receptors (Fas, TNFR1, etc.), mediates apoptosis and NF kappaB activation in many cells and tissues.
Apoptosis, a programmed cell death, is a operating process in normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by coupled of certain cytokines (TNF family - TNF, Fas ligand) and their death domain containing receptors (TNFR1, Fas receptor).

Applications:

Loading control

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Example Data:

Fig. 1. SDS-PAGE

Fig.1. SDS-PAGE Electrophoresis of TRAIL-R1 (DR4) / Fc recombinant protein (12% separating gel; reducing conditions).
Lane 1 to 3: TRAIL-R1 (DR4)/ Fc recombinant protein (2000 ng, 1000 ng, 500 ng per lane). Staining with Coomasie Blue.

Fig. 2. Western Blotting

Fig.2. Western Blotting of TRAIL-R1 (DR4) / Fc recombinant protein. Reducing conditions.
Lane 1 to 5: Immunostaining with TRAIL-R1 (DR4) specific antibody DR-4-02 (Cat. No. 10-403-C100; dilution 2 μg/ml ). Detection with Goat anti-mouse / HRP secondary antibody. Amount of TRAIL-R1 (DR4)/ Fc recombinant protein loaded on each lane: (1) 370 ng, (2) 150 ng, (3) 75 ng, (4) 37 ng, (5) 15 ng.

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Lyophilized

RL-001-C050

0.05 mg

390 EUR