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Wash Buffer
A buffer used to rinse ELISA plates between reagent addition steps
Improves Assays:
  • Reducing nonspecific background noise.
  • Increasing the specific signal-to-noise ratio.
How it Works:
Cell Technology's Wash Buffer is an optimal formulation of buffers and salts which maximizes the adsorption of antibodies and antigens onto polystyrene plates. During the adsorption process, the wash buffer stabilizes coated proteins, allowing for greater binding reactivity with the detection molecule, thereby enhancing the specific signal. By generating a higher specific signal, a lower concentration of coat protein may be needed when this buffer is used, thereby saving valuable reagents.
Directions:
Simply dilute the buffer 1:10 (100mL WB to 900mL diH20) and it's ready to use. To rinse microtiter plates, the buffer may be dispensed through a squirt bottle, a plate washer, or a multi-channel pipette. Be sure to fill the wells all the way up (400uL for a 96-well plate). We do NOT recommend submerging the entire plates in a bath as many of the wells become contaminated, which decreases the reliability of the assay.
Catalog Number
 		 Size (mL)
 		 Final volume after dilution
 		 Price
EWB100-1 100 1L €40.00
EWB100-2 500 5L €110.00
EWB100-3 1,000 10L €240.00
EWB100-4 10,000 100L €1,900.00